Stains for Electron Microscopy

Ammonium Molybdate
(Molybdic acid, ammonium salt)

Negative stain, used in negative staining viruses

Bismuth Metal
Granulated.

Used to prepare an EM stain for nucleic acids.

Albersheim & Killias, J.Cell Biol, 17, 93 (1963)
M.A.Hayat “Basic Techniques for TEM” p/184 (1986)

Gold Chloride,Hydrate

(Chloroauric Acid, Hydrogen Tetrachloroaurate)

Highly purified gold compound. Used for preparation of Colloidal Gold solutions.

 

CAS #	Chemical Formula	F.W
16961-25-4	H(AuCl4).H2O	357.79

Catalog #	Description	Qty
102	Gold Chloride	1 g
102-1	Gold Chloride	10 x 0.5 g /pkg
102-2	Gold Chloride	10 x 0.1 g / pkg
Please use the catalog number and description when ordering.

Ferric Chloride, Hexahydrate

Used to prepare positive and negative colloidal iron solutions as cell surface stains for EM.
 

CAS #	Chemical Formula	F.W
13478-10-9	FeCl3.6H2O	270.30

Catalog #	Description	Qty
102-10	Ferric Chloride, hexahydrate,	100 g
Please use the catalog number and description when ordering.

Indium Trichloride, EM
(Anhydrous)

A metal stain for nucleic acids.

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Lanthanum Nitrate

Used to prepare colloidal lanthanum
hydroxide-containing fixatives for
demonstration of intercellular spaces.
 

 

CAS #	Chemical Formula	F.W
10277-43-7	La(NO3)3.6H2O	433.03

Lead Acetate

Trihydrate, Reagent, A.C.S.

Metal stain for TEM. Used for in-block and thin sections staining
 

Lead Citrate

For the preparation of a simplified lead stain.
The most widely used metal stain for ultra thin sections.

Lead Nitrate

Reagent, A.C.S
 
A metal stain for ultra thin sections.
 

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Lead Tartrate

(Tartaric acid Lead (11) salt)
 

Methenamine

Hexamethylenetetramine, Hexamine
 
Used in conjunction with silver nitrate for staining carbohydrates.
 

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Methylamine Tungstate

An excellent negative stain. Unlike phosphotungstic acid it does not damage virus particles and it is consequently valuable for staining delicate viruses. The material wets grid films and specimens very well.
 

Phosphomolybdic acid

(dodeca-Molybdophosphoric acid)
 

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Phosphotungstic Acid

Crystal, Reagent Highest purity
(Tungstophosphoric acid)

Positive and negative stain. Also used as a fixative.
 

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Potassium Ferricyanide

Reagent, A.C.S
 

Potassium Permanganate

Reagent, A.C.S

EM Fixative and metal stain
 

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Crystal, Reagent, A.C.S.
 

Ruthenium Red 37.5% Ru

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Silver Nitrate
Reagent, A.C.S
 

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Sodium Citrate

Granular, Reagent
 

Sodium Borate, Reagent, A.C.S.

Borax
(Sodium Tetraborate, Decahydrate)
 

Sodium Silicotungstate

Valentine & Pereira’
J.Molec.Biol. 13, 13(1965)

Wilcox,Ginsberg & Anderson
J.Exp.Med. 118, 307

Sodium Tungstate

Negative stain.

Stockert J.C.Biol.Cellul. 29, 211(1977)
Takeuchi,I.K. J.Electron Micro., 30, 150(1981)
 

Thiocarbohydrazide (TCH)

Used in techniques for the demonstration of polysaccharides

and for the staining of membranes.

Seligman et al.,J.Cell Biol. 30,424(1966)
Seligman et al.J.Histochem.Cytochem.,13.629(1965)
Thiery,J.P. J.Microscopie., 6.987(1967)
Lo,H.K. et al.J.Histochem.Cytochem.35. 393 (1987)

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Thiosemicarbazide
Reagent, A.C.S.

Uranyl Acetate
Reagent, A.C.S.( Made from Depleted Uranium)

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Vanadyl Sulphate
(Vanadium (IV) oxide sulphate).
 
Used with ammonium molybdate for the preparation of vanadomolybdate stain.

Callahan & Homer, J.Cell Biol. 20, 350(1964)

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